In a joint statement reiterating their "outrage," the five leaders also said they were completely confident that the attempted assassination of former Russian double agent Sergei Skripal on British soil in March "was almost certainly approved at the governmental level" in Russia.
They also asked Moscow to open up with the organization for the Prohibition of Chemical Weapons (OPCW) on its Novichok program.
The leaders added that they would strengthen their activities to defend their societies against "malign state activity" and to interrupt the hostile actions of foreign intelligence networks.
"We, the leaders of France, Germany, the United States, Canada and the United Kingdom, reiterate our outrage at the use of a chemical nerve agent, known as Novichok, in Salisbury on March 4," they said in the statement issued in London. .
They welcomed the progress made in the investigation and the attempted murder charges brought against Russian suspects Alexander Petrov and Ruslan Boshirov, announced by Britain on Wednesday.
They also noted the OPCW findings that "the same chemical nerve agent" was used in the fatal poisoning of Dawn Sturgess. She was Charlie Rowley's girlfriend, who had picked up a bottle of fake perfume that contained Novichok.
"We urge Russia to provide full disclosure of its Novichok program to the OPCW," they said.
"We have full confidence in the British assessment that the two suspects were officers of the Russian military intelligence service, also known as GRU, and that this operation almost certainly was approved at the senior government level."
They said that the mass expulsion of undeclared GRU officers in the Russian embassies following the Salisbury attack had disrupted the service's activities.
"Yesterday's announcement further strengthens our intention to continue to disrupt the hostile activities of foreign intelligence networks in our territories, maintain the ban on chemical weapons, protect our citizens and defend ourselves against any form of malign state activity directed against us. and our societies. "
A Downing Street spokesman said Prime Minister Theresa May spoke with US President Donald Trump on Tuesday, Canadian leader Justin Trudeau on Wednesday and French President Emmanuel Macron and German Chancellor Angela Merkel on Thursday.