Phylogenetic tree showing the relationship between NCED3 proteins. Amino acid sequences were included in the phylogenetic analysis of the following species: Arabidopsis thaliana; Acer aretinum glycine max Medicago truncatula Asian rice; Phaseolus lunatus Phaseolus, vulgaris; Pisum sativum, Trifolium subterraneum. Vigna angularis, Vigna radiata, and Vigna unguiculata. Phylogenetic analysis was performed using MEGA-X, and a phylogenetic tree was generated by the neighbor-joining method with bootstrapping analysis (2000 repeats). credit: Plantarum physiology (2023). DOI: 10.1111/ppl.13943
Two MYB transcriptional regulators, FLP and the AtMYB88 homolog, recurrently regulate the homeostatic division of protective mother cells and the abiotic stress response in Arabidopsis thaliana. Only one orthologous gene of FLP has been identified in pea (Pisum sativum FLP; PsFLP).
Studying the development of stomata in peas is of great importance for improving some important agronomic traits in legumes. However, studies on the biological function of PsFLP in pea have not been reported.
In a study published in Plantarum physiologyInvestigators from the Xishuangbanna Tropical Botanical Garden (XTBG) of the Chinese Academy of Sciences and their collaborators investigated the oncogenic function of PsFLP using a virus-induced gene silencing (VIGS) technique.
By silencing PsFLP using the VIGS technique, the researchers obtained pea strains with abnormal clusters of stomata, suggesting that PsFLP may be involved in homozygous division of stomata. The number of stomata units per unit area was significantly higher in the silent strains than in the wild type.
Quantitative real-time PCR (qRT-PCR) data showed that PsFLP could inhibit the expression of CYCA2; 3 and CDKA; 1 and positively regulates the expression levels of AAO3, NCED3 and SnRK2.3 in Arabidopsis thaliana.
Thus, not only does PsFLP regulate homologous partitioning of stomata, but it may also regulate ABA synthesis and transduction genes, thus participating in drought stress response pathways.
“Our study provides new insights into improving plant stress tolerance by artificially modulating stomatal development,” said Professor Chen Jianhua of XTBG.
The results indicate that appropriately changing the number of stomata and adjusting the density of stomata can maintain the photosynthetic rate of plants and effectively prevent water loss, enhance drought resistance of plants, and improve crop yield.
more information:
Conghui Ning et al, An R2R3 The MYB transcription factor PsFLP regulates the replication division of guard mother cells during the development of stomata in Pisum sativum, Plantarum physiology (2023). DOI: 10.1111/ppl.13943
the quote: Artustryly modifying stomatal growth may improve crop stress tolerance (2023, June 7) Retrieved June 7, 2023 from https://phys.org/news/2023-06-artustryly-modulation-stomatal-crop-stress. html
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